Journal: Gastroenterology

Article Title: Toll-Like Receptor 9 Promotes Steatohepatitis by Induction of Interleukin-1 β in Mice

doi: 10.1053/j.gastro.2010.03.052

Figure Lengend Snippet: Kupffer cell–derived IL-1β promotes HSC activation. (A–D) HSCs isolated from WT, TLR9−/−, and IL-1R−/− mice were stimulated with 10 ng/mL IL-1β for 8 hours to measure mRNA expression of TIMP1 (A), PAI-1 (B), collagen (C), and Bambi (D) by quantitative real-time PCR (qPCR), and for 24 hours to determine TIMP1 expression in the supernatant by Western blot (A). (E) Kupffer cell–conditioned medium (Kup-CM) was prepared by treating Kupffer cells with 5 μg/mL CpG-ODN or non–CpG-ODN for 24 hours. Then, WT, TLR9−/−, and IL-1R−/− HSCs were treated with Kup-CM for 8 hours to determine mRNA expression of fibrogenic markers by qPCR (E, left) and for 24 hours to determine TIMP1 protein expression in the supernatant (E, right). Data represent mean ± SD; *P < .05, **P < .01; n.s., not significant.

Article Snippet: Blots were incubated with antibody for tissue inhibitor of metalloproteinase-1 (TIMP1; R&D Systems) or IL-1R–associated kinase M (IRAK-M; Cell Signaling Technology Inc, Danvers, MA).

Techniques: Derivative Assay, Activation Assay, Isolation, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Pharmaceutics

Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

doi: 10.3390/pharmaceutics17010138

Figure Lengend Snippet: Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).

Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: Pharmaceutics

Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

doi: 10.3390/pharmaceutics17010138

Figure Lengend Snippet: Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.

Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: Journal of Proteome Research

Article Title: Comparison of Protein Immunoprecipitation-Multiple Reaction Monitoring with ELISA for Assay of Biomarker Candidates in Plasma

doi: 10.1021/pr400877e

Figure Lengend Snippet: Response curves for IP-MRM analyses of recombinant TIMP1, COMP, MMP9, THBS2, MSLN and ENG proteins. Proteins were spiked at 10–640 ng/mL in a background matrix of 60 mg/mL BSA in DPBS and analyzed by IP-MRM as described in . Values plotted are mean ± standard deviation ( n = 3).

Article Snippet: TIMP1, ENG and MMP9 recombinant proteins were purchase from Sino Biological (Beijing, China).

Techniques: Recombinant, Standard Deviation

Journal: Journal of Proteome Research

Article Title: Comparison of Protein Immunoprecipitation-Multiple Reaction Monitoring with ELISA for Assay of Biomarker Candidates in Plasma

doi: 10.1021/pr400877e

Figure Lengend Snippet: Quantitation of six biomarker candidate proteins in plasma samples from colon cancer patients and noncancer controls by IP-MRM and ELISA. Plasma levels of TIMP1, COMP, MMP9, THBS2, MSLN and ENG were measured in controls (circles) and colon cancer patients (squares). Results are from the average of triplicate IP-MRM measurements and duplicate ELISA measurements.

Article Snippet: TIMP1, ENG and MMP9 recombinant proteins were purchase from Sino Biological (Beijing, China).

Techniques: Quantitation Assay, Biomarker Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Proteome Research

Article Title: Comparison of Protein Immunoprecipitation-Multiple Reaction Monitoring with ELISA for Assay of Biomarker Candidates in Plasma

doi: 10.1021/pr400877e

Figure Lengend Snippet: Correlation of protein expression levels measured by ELISA and IP-MRM in plasma samples. Mean concentrations from triplicate analyses of TIMP1, COMP, MMP9, THBS2, MSLN and ENG in plasma samples from 24 subjects (23 for TIMP1 and MMP9) by ELISA and IP-MRM are plotted. Pearson correlation coefficients are indicated in the figure.

Article Snippet: TIMP1, ENG and MMP9 recombinant proteins were purchase from Sino Biological (Beijing, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Proteome Research

Article Title: Comparison of Protein Immunoprecipitation-Multiple Reaction Monitoring with ELISA for Assay of Biomarker Candidates in Plasma

doi: 10.1021/pr400877e

Figure Lengend Snippet: Measurement summaries for biomarker candidates by IP-MRM assay and ELISA

Article Snippet: TIMP1, ENG and MMP9 recombinant proteins were purchase from Sino Biological (Beijing, China).

Techniques: Biomarker Assay, Enzyme-linked Immunosorbent Assay